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Mark K Hechinger

from Inglewood, CA

Also known as:
  • K Mark
  • Mark Kim Hechinger
  • M Hechinger
533 Hill St, Inglewood, CA 90302

Mark Hechinger Phones & Addresses

  • 533 Hill St, Inglewood, CA 90302
  • 1104 68Th St, Inglewood, CA 90302
  • Los Angeles, CA
  • Pasadena, CA

Work

  • Position:
    Sales Occupations

Education

  • Degree:
    Associate degree or higher

Emails

Business Records

Name / Title
Company / Classification
Phones & Addresses
Mark K. Hechinger
Director Of Laboratory
University of Southern California
Business/Secretarial School · College/University · Elementary/Secondary School
2250 Alcazar St, Los Angeles, CA 90089
323-4423204, 323-4423220, 323-4422920, 323-3421300

Us Patents

  • Platelet Immunoglobulin Bead Suspension And Flow Cytometry

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  • US Patent:
    6933106, Aug 23, 2005
  • Filed:
    Sep 13, 2002
  • Appl. No.:
    10/243515
  • Inventors:
    Mark Hechinger - Pasadena CA,
  • International Classification:
    G01N033/53
    G01N033/543
  • US Classification:
    435 4, 435 6, 435 71, 435 72, 435 793, 435 794, 435 795, 435 4051, 4351734, 435967, 435973, 436 10, 436 63, 436501, 436506, 436507, 436509, 436513, 436518, 436519, 436523, 436524, 436527, 436528, 436531, 436533, 436534, 436536, 436538, 436546, 436805, 436811
  • Abstract:
    Immunoassay methods and apparatus are provided which utilize flow cytometry, coated latex microspheres, and fluorochrome labeled antibodies, to simultaneously detect the presence and amount of one or more analytes in a sample. By combining FALS and fluorescence, it is practical to use beads of several different sizes, colors or shapes, each bead coated with a different analyte, for the simultaneous detection of one or more analytes and of cell components such as platelets in a sample.
  • Anti-Platelet Immunoglobulin Bead Positive Control

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  • US Patent:
    6951716, Oct 4, 2005
  • Filed:
    Jun 6, 2002
  • Appl. No.:
    10/163966
  • Inventors:
    Mark Hechinger - Pasadena CA,
  • International Classification:
    G01N033/53
    G01N033/543
  • US Classification:
    435 4, 435 6, 435 71, 435 72, 435 792, 435 793, 435 794, 435 795, 435967, 435973, 436 10, 436 63, 436501, 436506, 436507, 436509, 436513, 436518, 436519, 436523, 436524, 436527, 436528, 436531, 436533, 436534, 436536, 436538, 436546, 436805, 436811
  • Abstract:
    Immunoassay methods and apparatus are provided which utilize flow cytometry, coated latex microspheres, and fluorochrome labeled antibodies, to simultaneously detect the presence and amount of one or more analytes in a sample. Beads of several different sizes, colors or shapes, each bead are coated with a different analyte, for the simultaneous detection of one or more analytes and of cell components. The invention is also directed to platelet Ig positive control reagents and assays which provide for the setting of the fluorescence positive region for each patient. The platelet control is sized to fit between the platelets and red cells and thus making it ideal as a true biological control.
  • No Wash Bead Assay, Kit And Procedure

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  • US Patent:
    2002000, Jan 10, 2002
  • Filed:
    Jun 4, 2001
  • Appl. No.:
    09/873866
  • Inventors:
    Mark Hechinger - Pasadena CA,
  • International Classification:
    C12Q001/70
    G01N033/53
    G01N033/537
    G01N033/543
  • US Classification:
    435/005000, 435/007920, 436/518000
  • Abstract:
    A method of making a no wash bead based assay comprises preparing a first reagent comprising a buffer, and preparing a second reagent comprising a protein. Beads of preselected size and having a coefficient of variation less than 5% are prepared, including washing the beads in the buffer to form a bead-buffer matrix and reducing the surfactancy of the beads to an effective amount. Thereafter, an antigen for detecting the presence of a target species is added to the bead-buffer matrix such that the antigen attaches to the beads to form a bead-antigen mixture. The surfactancy of the beads facilitates attachment of the antigen thereto. Buffer is added to the bead-antigen mixture and thereafter the mixture is incubated. The second reagent is added to the bead-antigen mixture to reduce or eliminate non-specific binding sites.
  • Immunoassay Apparatus, Kit And Methods

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  • US Patent:
    2003000, Jan 9, 2003
  • Filed:
    Dec 12, 2001
  • Appl. No.:
    10/023434
  • Inventors:
    Mark Hechinger - Pasadena CA,
  • International Classification:
    G01N021/76
  • US Classification:
    436/172000
  • Abstract:
    Immunoassay methods and apparatus are provided which utilize flow cytometry, coated latex microspheres, and fluorochrome labeled antibodies, to simultaneously detect the presence and amount of several antigens or antibodies in a sample. The use of microspheres, beads, or other particles as solid supports for antigen-antibody reactions in order to detect antigens or antibodies in serum and other body fluids is particularly attractive when linked to flow cytometry. Flow cytometers have the capacity to detect particle size differences and are highly sensitive fluorescence detectors. It is practical to use beads of several different sizes, colors or shapes, each bead coated with a different protein or antibody, for the simultaneous detection of multiple analytes in a sample.
  • One-Step, No-Wash Multiplex Bead-Based Flow Cytometric Assay

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  • US Patent:
    2005010, May 19, 2005
  • Filed:
    Sep 24, 2004
  • Appl. No.:
    10/949938
  • Inventors:
    Mark Hechinger - Inglewood CA,
  • International Classification:
    C12Q001/70
  • US Classification:
    435005000
  • Abstract:
    A no-wash multiplex bead system is disclosed in which all basic reaction materials can be placed into single container via manual application, robotic transfer, or any other batch-type testing procedure and allowed to incubate followed by analysis on a flow cytometer.
  • No-Wash Bead Assay, Kit And Procedure

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  • US Patent:
    2005022, Oct 6, 2005
  • Filed:
    Jun 3, 2005
  • Appl. No.:
    11/144860
  • Inventors:
    Mark Hechinger - Inglewood CA,
  • International Classification:
    C12Q001/68
    G01N033/543
  • US Classification:
    435006000, 436523000
  • Abstract:
    A method of making a no wash bead based assay comprises preparing a first reagent comprising a buffer, and preparing a second reagent comprising a protein. Beads of preselected size and having a coefficient of variation less than 5% are prepared, including washing the beads in the buffer to form a bead-buffer matrix and reducing the surfactancy of the beads to an effective amount. Thereafter, an antigen for detecting the presence of a target species is added to the bead-buffer matrix such that the antigen attaches to the beads to form a bead-antigen mixture. The surfactancy of the beads facilitates attachment of the antigen thereto. Buffer is added to the bead-antigen mixture and thereafter the mixture is incubated. The second reagent is added to the bead-antigen mixture to reduce or eliminate non-specific binding sites.

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    • Friends:
      Manny Hornedo, Mike Lyons, Michael Cosgrove, Tom-Sue Schneider Armona

Classmates

Mark Hechinger Photo 2

Mark Hechinger

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Schools:
Manchester Regional High School Haledon NJ 1968-1972
Community:
Karen Huther, Ron Pluymers, John Ajjan, Michael Sands, Tony Alvarez, Eugene Cascio, William Garner

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